A Fully Automated Wirelessly Powered Centrifugal Platform towards a Sample-to-answer Chemiluminescent Elisa Assay for Cvd Detection
نویسندگان
چکیده
This paper describes the development of a platform where a silicon photo-multiplier (SiPM) sensor co-rotates with a “Lab-on-a-Disc” (LoaD). This platform includes a modular data-logger and, to avoid the need for electrical slip rings, it is wirelessly powered. This system is optimized for the detection of chemiluminescence. It is characterized on-disc using dilutions of HRP tagged antibodies and shows good agreement with a commercial luminometer. Additionally, preliminary work towards developing a fully integrated on-disc ELISA assay for CVD detection is presented.
منابع مشابه
A Fully Integrated Multiplexed Immunoassay on a Disc
A fully automated lab-on-a-disc for simultaneous detection of multiple protein biomarkers in raw samples such as whole blood or whole saliva is developed. Based on the bead-based sandwich-type Enzyme-linked immunosorbent assay (ELISA), here a novel centrifugal microfluidic layout was designed and tested to accomplish the simultaneous detection of C-reactive protein (hsCRP), cardiac troponin I (...
متن کاملCentrifugal Microfluidic Platform for Ultrasensitive Detection of Botulinum Toxin
We present an innovative centrifugal microfluidic immunoassay platform (SpinDx) to address the urgent biodefense and public health need for ultrasensitive point-of-care/incident detection of botulinum toxin. The simple, sample-to-answer centrifugal microfluidic immunoassay approach is based on binding of toxins to antibody-laden capture particles followed by sedimentation of the particles throu...
متن کاملPerformance characteristics of three automated immunoassays for thyroid hormones
Background: Since the introduction of the first radioimmunoassay, several improvements have been made in the design of immunoassays such as method of antibody production, labeling, automation and detection technology. We performed an analytical evaluation of the new electrochemiluminescent immunoassay (ECLIA) for serum TSH, FT4 and T3 in the Elecsys 2010 immunoassay system and compared the resu...
متن کاملRapid, automated quantification of total human chorionic gonadotropin in serum by a chemiluminescent enzyme immunometric assay.
Total human chorionic gonadotropin (hCG-hCG beta) in serum was assayed with the immulite system, a fully automated random-access immunoassay analyzer that has a unique centrifugal procedure for solid-phase washing and a chemiluminescent detection system. The broad range of the hCG calibration curve (up to 5000 IU/L) is achieved by using a small serum sample size (5 microL), which provides suffi...
متن کاملEvaluation of three commercial serological tests with different methodologies to assess Helicobacter pylori infection.
The sera of 142 Helicobacter pylori-positive and 32 H. pylori-negative patients were assessed by a desktop test (QuickVue), an enzyme-linked immunosorbent assay (ELISA) (HM-CAP), and a solid-phase, two-step chemiluminescent enzyme immunoassay (Immulite). These tests yielded sensitivities of 97, 97, and 91% and specificities of 97, 94, and 100%, respectively. In conclusion, the desktop test and ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2016